RA/Sr. RA, Antibody Generation

Prellis Biologics uses proprietary technology to 3D print lymph node organoids, which we are using to generate in vitro antibody libraries andinvitrohumanlymphnodecoculturesfor generating antigen-specific B-cells.We are seeking a Research Associate – Senior Research Associate, Antibody Generation to join our Antibody Screening team.This role will report to the Director of Antibody Screening with accountability for supporting high-throughput antibody discovery efforts, particularly using single B-cell approaches.The ideal candidate will have a strong background in molecular biology, with significant experience in supporting end-to-end antibody discovery campaigns.

Key Responsibilities:

• Perform high-throughput molecular biology techniques, including but not limited to, PCR, molecular cloning, and plasmid preparation to generate recombinant antibodies.
• Support high-throughput antibody sequencing to drive antibody discovery projects.
• Operate and maintain liquid-handling robots and other lab automation platforms to facilitate large-scale sequencing and cloning workflows.
• Maintain cell lines using aseptic techniques.Perform transfections for high-throughput antibody expression.
• Support high-throughput protein purification processes.
• Independently troubleshoot and contribute to protocol optimization for enhanced efficiency and increased throughput.
• Assist in the development and optimization of automation protocols for improved efficiency in molecular and cellular assays.
• Collaborate with cross-functional teams, providing technical support for various projects requiring automated workflows.
• Maintain accurate records of experiments, analyze data, and present results in team meetings.

Qualifications:

• B.S in Molecular Biology, Immunology, Biotechnology, or related life science field with 4+ years of experience, or M.S. with 2+ years of relevant experience.
• Strong proficiency in NGS workflows, cloning techniques, and protein expression and purification methods.
• Experience with liquid-handling robots and other automation equipment is required.
• Ideal candidates will have prior experience in single B-cell antibody discovery workflows with an end goal of identifying novel therapeutic candidates.
• Strong organizational and time management skills to contribute to multiple projects in a fast paced environment.
• Excellent communication skills and ability to document and present experimental results.
• Familiarity with bioinformatics tools for NGS data analysis is a plus.